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Determination of fat-soluble component in Radix Salviae miltiorrhizae from different sources

Rui An

Open publisher page 2 citations

Abstract

AIM: To study the contents and determination method of dihydrotanshinone I, cryptotanshinone, tanshinone I, tanshinone IIA in Danshen from different sources. METHODS: Determination by HPLC with the gradient eluent consisted of methanol (1% acetic acid) water (1% acetic acid). The detection wavelength was at 280 nm. RESULTS: The average recovery of four components were all above 97%, RSD was no more than 1.2%. The calibration curve was linear. CONCLUSION: The method is accurate and reliable with good reproducibility.

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What this paper is about

AIM: To study the contents and determination method of dihydrotanshinone I, cryptotanshinone, tanshinone I, tanshinone IIA in Danshen from different sources. METHODS: Determination by HPLC with the gradient eluent consisted of methanol (1% acetic acid) water (1% acetic acid). The detection wavelength was at 280 nm. RESULTS: The average recovery of four components were all above 97%, RSD was no more than 1.2%. The calibration curve was linear. CONCLUSION: The method is accurate and reliable with good reproducibility.

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Available abstract

AIM: To study the contents and determination method of dihydrotanshinone I, cryptotanshinone, tanshinone I, tanshinone IIA in Danshen from different sources. METHODS: Determination by HPLC with the gradient eluent consisted of methanol (1% acetic acid) water (1% acetic acid). The detection wavelength was at 280 nm. RESULTS: The average recovery of four components were all above 97%, RSD was no more than 1.2%. The calibration curve was linear. CONCLUSION: The method is accurate and reliable with good reproducibility.

Key concepts: Radix (gastropod), Chromatography, Chemistry, Reproducibility, Acetic acid, Calibration curve, High-performance liquid chromatography, Column chromatography

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