Quantitative determination of 3 fat-soluble components in radix salviae miltiorrhizae from different sources and species by RP-HPLC

To use the method of ultrasonic extraction to extract the fat-soluble components in radix salviae miltiorrhizae,the determination of cryptotanshinone,tanshinone I and tanshinone ⅡA by RP-HPLC.The column was Diamonsil TM C_ 18(250×4.6mm,5μm);the mobile phase was methanol-water(75∶25),the flow rate was 0.8mL/min,the detection wavelength was 270nm.The calibration curves of cryptotanshinone,tanshinone I,tanshinone ⅡA were linear in the range of 1.456～10.19,2.160～15.12 and 3.152～22.06μg/mL with the correlation coefficient beyond 0.9991,0.9992 and 0.9996 respectively.The mean recoveries of the assay were 98.04%(RSD=1.3%,n=3) for cryptotanshinone,99.65%(RSD=1.2%,n=3) for tanshinone I,and 99.31%(RSD=1.2%,n=3) for tanshinone ⅡA respectively.The method of quantitative control was rapid,accurcy and reliability,and can be used to control the quality of radix salviae miltiorrhizae.