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HPLC Determination of Four Tanshinones in Radix Salviae

Haiqing Liu, Lu Dong, Chen Yuerang, Wang Huichun, Yang Feng-mei, Xining

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Abstract

Objective:To establish an HPLC method for the determination of four tanshiones in Radix Salviae.Methods:The column was Elite-ODS(5 μm, 150 mm×4.6mm) ; the mobile phase consisted of a mixture of methanol-water(75:25),the column temperature was 22℃ ,the flow rate was 1.0 mL·min~(-1) and the detection was done at 270 nm.Results:The average recovery of tanshinone Ⅱ_A,tanshinone I,cryptotanshinone and dihydrotanshinone I were 100.6%,100.0%,94.5% and 96.6% (RSD=0.9%,1.1%,1.8% and 1.4%,n=3).Conclusion:It was also shown from HPLC the difference in the distributions and contents in Radix Salviae from difficult place.

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What this paper is about

Objective:To establish an HPLC method for the determination of four tanshiones in Radix Salviae.Methods:The column was Elite-ODS(5 μm, 150 mm×4.6mm) ; the mobile phase consisted of a mixture of methanol-water(75:25),the column temperature was 22℃ ,the flow rate was 1.0 mL·min~(-1) and the detection was done at 270 nm.Results:The average recovery of tanshinone Ⅱ_A,tanshinone I,cryptotanshinone and dihydrotanshinone I were 100.6%,100.0%,94.5% and 96.6% (RSD=0.9%,1.1%,1.8% and 1.4%,n=3).Conclusion:It was also shown from HPLC the difference in the distributions and contents in Radix Salviae from difficult place.

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Available abstract

Objective:To establish an HPLC method for the determination of four tanshiones in Radix Salviae.Methods:The column was Elite-ODS(5 μm, 150 mm×4.6mm) ; the mobile phase consisted of a mixture of methanol-water(75:25),the column temperature was 22℃ ,the flow rate was 1.0 mL·min~(-1) and the detection was done at 270 nm.Results:The average recovery of tanshinone Ⅱ_A,tanshinone I,cryptotanshinone and dihydrotanshinone I were 100.6%,100.0%,94.5% and 96.6% (RSD=0.9%,1.1%,1.8% and 1.4%,n=3).Conclusion:It was also shown from HPLC the difference in the distributions and contents in Radix Salviae from difficult place.

Key concepts: Chemistry, Radix (gastropod), Chromatography, High-performance liquid chromatography, Methanol, Organic chemistry, Biology, Botany

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