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2004•Shandong Agricultural SciencesRequires access

Study on purity testing methods for maize hybrids using SSR markers

Zhen Wah Tan

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Abstract

In this study, 9 SSR primer pairs were screened to search for the SSR loci that are suitable for testing the purities of 4 maize hybrids, which lack a reliable laboratory method for detecting a large proportion of variation in SSR allele sizes. Polymorphic SSR loci have been found that could be applied to test the purities of these 4 hybrids. A procedure featuring inexpensive equipment and ease of use has been set up for testing maize hybrid purity using SSR marker. The feasibility of applying SSR markers to maize hybrid purity using this procedure was discussed .

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What this paper is about

In this study, 9 SSR primer pairs were screened to search for the SSR loci that are suitable for testing the purities of 4 maize hybrids, which lack a reliable laboratory method for detecting a large proportion of variation in SSR allele sizes. Polymorphic SSR loci have been found that could be applied to test the purities of these 4 hybrids. A procedure featuring inexpensive equipment and ease of use has been set up for testing maize hybrid purity using SSR marker. The feasibility of applying SSR markers to maize hybrid purity using this procedure was discussed .

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Available abstract

In this study, 9 SSR primer pairs were screened to search for the SSR loci that are suitable for testing the purities of 4 maize hybrids, which lack a reliable laboratory method for detecting a large proportion of variation in SSR allele sizes. Polymorphic SSR loci have been found that could be applied to test the purities of these 4 hybrids. A procedure featuring inexpensive equipment and ease of use has been set up for testing maize hybrid purity using SSR marker. The feasibility of applying SSR markers to maize hybrid purity using this procedure was discussed .

Key concepts: Hybrid, Biology, Microsatellite, Primer (cosmetics), Biotechnology, Allele, Genetics, Agronomy

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