The Relativity between SSR Method and Field Test in the Hybrids Purity Identification of Maize

To establish rapid and accurate purity detection technique is effective measure for maize seed purity controlling.The paper aims to analysis the factors that lead to the differences between SSR identification and field test,and to present the relevant solutions.The SSR primer pairs,Umc1153 and phi072,were used to screen 300 individuals of stock seed,field inbred and hybrids respectively,and to detect 16 hybrid samples of Lainong 14 and Lainong 15.The detection rate of the specific polymorphisms reduced progressively along with generations might be one factor that influences the difference between SSR and field test.And emergence rate was another important factor.Finally,regression equations were built between the SSR identification and field test,which was useful to correcting the result of SSR identification.