2007Anhui nongye kexueRequires access

Study on the Establishment of Rieger Begonia Regeneration System

Zhang Yun-feng

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Abstract

Plant regeneration system from Rieger begonia leaf culture was studied and established.The results showed that petiole Rieger begonia leaf with main vein(1 cm2) was used as explants;the optimum medium for adventitious shoot induction was MS + 6-BA 0.5 mg/L + NAA 0.5 mg/ L or MS + 6-BA 0.5 mg/L + NAA 0.1 mg/ L + 4% aloe juice,and abundant shoots were obtained after 30~40 d from pre-cultured explants on the medium.MS + 6-BA 0.05 mg/L + NAA 0.1 mg/L and 1/2MS + IBA 0.5 mg/L was suitable medium for shoot multiplication and rooting,respectively.

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What this paper is about

Plant regeneration system from Rieger begonia leaf culture was studied and established.The results showed that petiole Rieger begonia leaf with main vein(1 cm2) was used as explants;the optimum medium for adventitious shoot induction was MS + 6-BA 0.5 mg/L + NAA 0.5 mg/ L or MS + 6-BA 0.5 mg/L + NAA 0.1 mg/ L + 4% aloe juice,and abundant shoots were obtained after 30~40 d from pre-cultured explants on the medium.MS + 6-BA 0.05 mg/L + NAA 0.1 mg/L and 1/2MS + IBA 0.5 mg/L was suitable medium for shoot multiplication and rooting,respectively.

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Available abstract

Plant regeneration system from Rieger begonia leaf culture was studied and established.The results showed that petiole Rieger begonia leaf with main vein(1 cm2) was used as explants;the optimum medium for adventitious shoot induction was MS + 6-BA 0.5 mg/L + NAA 0.5 mg/ L or MS + 6-BA 0.5 mg/L + NAA 0.1 mg/ L + 4% aloe juice,and abundant shoots were obtained after 30~40 d from pre-cultured explants on the medium.MS + 6-BA 0.05 mg/L + NAA 0.1 mg/L and 1/2MS + IBA 0.5 mg/L was suitable medium for shoot multiplication and rooting,respectively.

Key concepts: Begonia, Explant culture, Shoot, Petiole (insect anatomy), Botany, Murashige and Skoog medium, Regeneration (biology), Biology

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