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2010•Chinese Journal of Modern Drug ApplicationRequires access

Determination of Quercetin Contents in Fukean Tablet by HPLC

Rong Shu-yin

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Abstract

Objective To establish a HPLC method for determination of Quercetin Contents in Fukean Tablet. Methods The column was Diamonsil C18 ( 150 × 4. 6mm,5 μ) . The mobile phase was a mixture of methanol and 0. 4% phosphoric acid solution( 52∶ 48) at a flow rate of 1. 0 ml/min,and the detecting wavelength was at 360 nm,the temperature was 35℃. Results The linear range of Baicalin was 0. 2 ～ 2. 0μg( r = 0. 9999) and the average recovery rate was 99. 88% ( RSD = 0. 93% ) . Conclusion The method is simple,high sensitiv-ity and accurate with good repeatability and can be used for quality control in Fukean Tablet.

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What this paper is about

Objective To establish a HPLC method for determination of Quercetin Contents in Fukean Tablet. Methods The column was Diamonsil C18 ( 150 × 4. 6mm,5 μ) . The mobile phase was a mixture of methanol and 0. 4% phosphoric acid solution( 52∶ 48) at a flow rate of 1. 0 ml/min,and the detecting wavelength was at 360 nm,the temperature was 35℃. Results The linear range of Baicalin was 0. 2 ～ 2. 0μg( r = 0. 9999) and the average recovery rate was 99. 88% ( RSD = 0. 93% ) . Conclusion The method is simple,high sensitiv-ity and accurate with good repeatability and can be used for quality control in Fukean Tablet.

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Available abstract

Objective To establish a HPLC method for determination of Quercetin Contents in Fukean Tablet. Methods The column was Diamonsil C18 ( 150 × 4. 6mm,5 μ) . The mobile phase was a mixture of methanol and 0. 4% phosphoric acid solution( 52∶ 48) at a flow rate of 1. 0 ml/min,and the detecting wavelength was at 360 nm,the temperature was 35℃. Results The linear range of Baicalin was 0. 2 ～ 2. 0μg( r = 0. 9999) and the average recovery rate was 99. 88% ( RSD = 0. 93% ) . Conclusion The method is simple,high sensitiv-ity and accurate with good repeatability and can be used for quality control in Fukean Tablet.

Key concepts: Repeatability, Baicalin, Chromatography, Phosphoric acid, Chemistry, Quercetin, High-performance liquid chromatography, Linear range

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