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Determination of Quercetin in Duizuoye Tablet by HPLC

Lan Ying-ni

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Abstract

OBJECTIVE: To establish an HPLC method for the determination of Quercetin in Duizuoye tablet. METHODS: HPLC analysis was carried out on Dikma-ODS C18 column (250 mm×4.6 mm, 5 μm) with methanol-0.4% phosphoric acid solution(53∶47) as mobile phase at a flow rate of 1.0 mL·min-1; the detection wavelength was set at 370 nm and the column temperature was maintained at 35 ℃. RESULTS: The calibration curve for Quercetin was linear in the range of 1.31～9.17 μg·mL-1 (r=0.999 8).The average recovery was 98.06%, with RSD of 1.05% (n=6).CONCLUSION: The adopted method is simple, rapid and accurate, and suitable for the quality control of Duizuoye tablet.

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What this paper is about

OBJECTIVE: To establish an HPLC method for the determination of Quercetin in Duizuoye tablet. METHODS: HPLC analysis was carried out on Dikma-ODS C18 column (250 mm×4.6 mm, 5 μm) with methanol-0.4% phosphoric acid solution(53∶47) as mobile phase at a flow rate of 1.0 mL·min-1; the detection wavelength was set at 370 nm and the column temperature was maintained at 35 ℃. RESULTS: The calibration curve for Quercetin was linear in the range of 1.31～9.17 μg·mL-1 (r=0.999 8).The average recovery was 98.06%, with RSD of 1.05% (n=6).CONCLUSION: The adopted method is simple, rapid and accurate, and suitable for the quality control of Duizuoye tablet.

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Available abstract

OBJECTIVE: To establish an HPLC method for the determination of Quercetin in Duizuoye tablet. METHODS: HPLC analysis was carried out on Dikma-ODS C18 column (250 mm×4.6 mm, 5 μm) with methanol-0.4% phosphoric acid solution(53∶47) as mobile phase at a flow rate of 1.0 mL·min-1; the detection wavelength was set at 370 nm and the column temperature was maintained at 35 ℃. RESULTS: The calibration curve for Quercetin was linear in the range of 1.31～9.17 μg·mL-1 (r=0.999 8).The average recovery was 98.06%, with RSD of 1.05% (n=6).CONCLUSION: The adopted method is simple, rapid and accurate, and suitable for the quality control of Duizuoye tablet.

Key concepts: Phosphoric acid, Chromatography, High-performance liquid chromatography, Calibration curve, Quercetin, Chemistry, Content determination, Linear range

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