Establishment and evaluation of method of HPLC for determination of quercetin and kaempferol in Qianliebeixi tablet

Objective To establish the method of high performance liquid chromatography(HPLC) for determining quercetin and kaempferol in Qianliebeixi tablet,and provide basis for the quality control of industrial production.Methods The method of HPLC was developed to determine quercetin and kaempferol in Qianliebeixi tablet.The developed conditions of HPLC were as follows:the shim-pack VP-ODS C18(150.0 mm×4.6 mm,5 μm),Acetonitrile-0.2% phosphoric acid solution(30∶70) mobile phase,370 nm detection wave length,and 30℃ column temperature.Results The linear range of quercetin was 0.042 6-0.639 0 μg,the regression equation was Y=-10 931+1 426 905X,r=0.999 8,the average recovery was 98.5%,and the RSD was 2.61%(n=6).The linear range of kaempferol was 0.045 4~0.726 4 μg,the regression equation was Y=-55 868+1 656 625X,r=0.999 2,the average recovery was 100.3%,and the RSD was 2.28%(n=6).The RSD in accuracy,stability and repeatability test were 1.66%,2.11%,and 1.35%,respectively.The content of quercetin or kaempferol in Qianliebeixi tablet was limited not less than 0.7 mg per one tablet under the developed condition.Conclusion The method of HPLC for determining quercetin and kaempferol in Qianliebeixi tablet is accurate,simple and reliable,which provides the basis for the quality control of Qianliebeixi tablet.