Simultaneous Determination of Kaempferol and Quercetin in Euphorbia sororia by HPLC

OBJECTIVE:To establish a method for the simultaneous determination of kaempferol and quercetin in Euphorbia sororia.METHODS:HPLC method was adopted.Hypersil ODS-2 C18 column(250 mm×4.6 mm,5 μm)was used with mobile phase consisted of methanol-0.4% phosphonic acid solution(52∶48,V/V)at the flow rate of 1.0 ml/min.The detection wavelength was set at 360 nm,and column temperature was 30 ℃.RESULTS:The linear ranges of kaempferol and quercetin were 5.2-156.0 μg/ml(r=0.999 9)and 3.1-92.1 μg/ml(r=0.999 7),respectively.RSDs of precision,reproducibility and stability were all lower than 3%.The average recoveries were 101.73%(RSD=1.27%,n=6)and 101.39%(RSD=1.59%,n=6),respectively.CONCLUSIONS:The method is quick,simple,rapid and reproducible for the determination of kaempferol and quercetin in E.sororia.