Determination of Quercetin and Kaempferol in Herba Orostachgdis Fimbriati by HPLC Isocratic Elution

Objective: To establish an HPLC method for determination quercetin,kaempferol in Herba Orostachgdis Fimbriati. Method: The separation was performed on Thermo C18( 4.6 mm × 250 mm,5 μm)column. The mobile phase consisted of methanol-acetate buffer solution( 53∶ 47) with the flow rate of 1. 0 mL·min-1,the column temperature was 30 ℃,and the detection wavelength was 360 nm,sample size was 20 μL.Result: The quercetin,kaempferol were linear in the ranges of 3. 92-11. 76 mg·L-1( r = 0.999 8),10.03-30. 09 mg ·L-1( r = 0. 999 9),respectively. The average recoveries were 99.85%,100.07%( n = 9),respectively. RSDs were 0. 78%,1. 05%,respectively. Conclusion: The method is simple,accurate and reproducible,and can be used for the quality control of Herba Orostachgdis Fimbriati.