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2013•Journal of Inner Mongolia Medical UniversityRequires access

SIMULTANEOUS DETERMINATION OF QUERCETIN AND KAEMPFEROL IN ERITRICHII HERBA BY HPLC

Lijuan Ma

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Abstract

Objective:To establish a determination method of quercetin and kaempferol in Eritrichii Herba.Methods:HPLC was applied.The column was Eighteen alkyl silane bonded silica gel,the mobile phase was methanol~0.4%phosphoric acid(45∶ 50),the flow rate was 1.0 mL/ min and the detection wavelength was 368nm.Result:The linear range of Quercetin was 31.4~314 ng(r=0.9997),the average recovery was 96.96%(RSD=1.54%).The linear range of kaempferol was 30~300 ng(r=0.9999),the average recovery was 97.92%(RSD=1.91%).Conclusion:The method is simple,accurate,reproducible and can be used for the determination of quercetin and kaempferol in Eritrichii Herba.

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What this paper is about

Objective:To establish a determination method of quercetin and kaempferol in Eritrichii Herba.Methods:HPLC was applied.The column was Eighteen alkyl silane bonded silica gel,the mobile phase was methanol~0.4%phosphoric acid(45∶ 50),the flow rate was 1.0 mL/ min and the detection wavelength was 368nm.Result:The linear range of Quercetin was 31.4~314 ng(r=0.9997),the average recovery was 96.96%(RSD=1.54%).The linear range of kaempferol was 30~300 ng(r=0.9999),the average recovery was 97.92%(RSD=1.91%).Conclusion:The method is simple,accurate,reproducible and can be used for the determination of quercetin and kaempferol in Eritrichii Herba.

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Available abstract

Objective:To establish a determination method of quercetin and kaempferol in Eritrichii Herba.Methods:HPLC was applied.The column was Eighteen alkyl silane bonded silica gel,the mobile phase was methanol~0.4%phosphoric acid(45∶ 50),the flow rate was 1.0 mL/ min and the detection wavelength was 368nm.Result:The linear range of Quercetin was 31.4~314 ng(r=0.9997),the average recovery was 96.96%(RSD=1.54%).The linear range of kaempferol was 30~300 ng(r=0.9999),the average recovery was 97.92%(RSD=1.91%).Conclusion:The method is simple,accurate,reproducible and can be used for the determination of quercetin and kaempferol in Eritrichii Herba.

Key concepts: Kaempferol, Quercetin, Phosphoric acid, Chromatography, Chemistry, High-performance liquid chromatography, Linear range, Detection limit

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