Simultaneous Determination of 3 Components in Cuscuta chinensis by HPLC

OBJECTIVE:To establish a method for simultaneous determination of quercetin,hyperoside and kaempferol from Cuscuta chinensis. METHODS:HPLC method was adopted. The separation was performed on Hypersil ODS-2(300 mm×4.6 mm, 5 μm)column with mobile phase consisted of methanol-water(58∶42,V/V). The detection wavelength was set at 281 nm and column temperature was 30 ℃. The flow rate was 1.0 ml/min. RESULTS:The linear ranges of quercetin,hyperoside and kaempferol were 10-50 μg/ml(r=0.999 7),2.5-30 μg/ml(r=0.999 5),2.5-30 μg/ml(r=0.999 6),respectively. RSDs of precision,stability and reproducibility tests were all lower than 2%. The average recoveries of three active compounds were 98.90%(RSD=1.23%,n=6), 97.60%(RSD=1.37%,n=6)and 97.39%(RSD=1.40%,n=6). CONCLUSIONS:The method is simple,rapid and accurate, and provides reference for quality evaluation of C. chinensis.