Comparison of two cell lines for cell model of hepatocytic steatosis in vitro

Objective To compare two models of hepatocytic steatosis in vitro,which were established by treating hepatocarcinoma cell HepG2 and human liver cell L02 with oleic acid in vitro.Through examination the role of compound methionine and choline bitartrate,to find a convenient and stable model in vitro for drug screening.Methods Normal group,HepG2 model group,groups of HepG2 cells treated with compound methionine and choline bitartrate at different concentrations,L02 model group,and model groups of L02 cells treated with drug at different concentratrions were set up.The hepatocytic steatosis models were established by treating HepG2 and L02 with oleic acid in vitro.The fatty droplets in cells could be observed by oil red O staining under light microscope.ALT,AST,and γ-GT in cell supernatant were detected by reactant kits.Results After treated with oleic acid for 24 hours,the fatty droplets in cells were observed by oil red O staining under light microscope.With the prevention with compound methionine and choline bitartrate,the amount of lipid droplet in HepG2 cells did not decrease,but decreased in L02 cells after treated with oleic acid for 48 hours.Compared with the model group,the integral optical density(IOD) value of positive of red fatty droplets in the group treated with drugs decreased markedly(P0.01).There were significant differences in ALT,AST,and γ-GT in cell supernatant in the groups treated with