Detected Results of Tumor Markers Using Protein Biochip Compared with ELISA Method

Objective To evaluate the detected difference of tumor markers between protein biochip and ELISA method.Methods The serum levels of 3 common used tumor markers, including AFP, CA199, and CEA, were measured with the C-12 protein biochip detective system in 50 primary hepatic cancer patients, 17 patients with liver cirrhosis, 16 patients with chronic hepatitis and 40 healthy persons. Meanwhile, the 3 tumor markers serum levels were also detected by ELISA.Results Combined measured positive rate and specificity for PHC were 78.00% and 82.19% when the 3 tumor markers were measured with protein biochip, but the positive rate and specificity were 78.00% and 75.34% by ELISA. There wasn't significant difference between these two methods(P0.05).The coincident rate was 92.95%, and spearman rank correlation was 0.842(P0.001).Conclusion Protein biochip could be measured the serum tumor markers levels accurately, and it was more quickly and conveniently than traditional methods. It could be used as a common means for the measurement of tumor markers.