Influence of platelet-rich plasma (PRP) on alkaline phosphate activity and total protein concent of bone marrow stromal cells (BMSCs)

Objective:In vitro to evaluate the effects of PRP on alkaline phosphate (ALP) activity and total protein content of BMSCs.Method: Immunochemical staining, kinetic method and Coomassie brilliant blue staining were used in this study.Result:The PRP group and the osteogenic-differentiating medium group showed strongly positive immunochemical staining while the control group showed negative. Similar to staining above, the alkaline phosphate activity means were significantly different between the PRP group and the control group (P0.01) and there was no difference between the PRP group and the osteogenic-differentiating medium group (P0.05). As to total protein content, even though the content of PRP group was lower than that of osteogenic-differentiating medium group, the statistical significance existed between the two experimental groups and the control group. Conclusion: PPR promotes alkaline phosphate activity and total protein content toward bone marrow stromal cells.