Effects of platelet-rich plasma on mineral characteristics of bone marrow stromal cells

Objective To investigate the effects of platelet-rich plasma(PRP) on the alkaline phosphatase(ALP) activity and mineralized nodule formation of dog's bone marrow stromal cells(MSCs). Methods Various concentrations of PRP(0,10%,20%,30%) and rhBMP-2 were added to the medium of cultured third passage MSCs. After 3,6,9,12 days of co-incubation, the ALP activity was measured. After the third passage MSCs was cultured for 4 weeks, mineralized solution and PRP(10%,30%) were added into the test group medium. Mineralized solution was added into the medium of the control group. At day 28,the formation of mineralized nodules was detected with Dahl McGec-Russll's alizarin red stain and von Kossa's silver nitrate for calcium. Results PRP at the concentration of 10% could increase ALP activity; the 10%PRP had an optimal effect(P0.05).PRP could also induce nodule formation to some degree. Conclusion PRP has the tendency of inducing MSCs to differentiate into osteoblastic cells.