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2004•Journal of Shandong University of Traditional Chinese MedicineRequires access

HPLC DETERMINATION OF PUERARIN IN GELIAN ZHIXIE GRANULE

Li Jun

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Abstract

Objective: To establish a HPLC method for the determination of puerarin in Gelian Zhixie granule. Methods: The chromatographic conditions were Beckman ODS C 18 colum,mobile phrase of methonal -2.5% acetic acid (25∶75),flow rate of 1.0 ml·min -1 and detection wavelength at 250 nm. Results: The standard curve was linear over the range of 0.1～1 μg with the correlation coefficient 0.9998.The average recovery was 98.64% with RSD=1.72%(n=5). Conclusion: The HPLC method is sensitive, rapid and accurate. It can be used to control the quality of Gelian Zhixie granule.

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What this paper is about

Objective: To establish a HPLC method for the determination of puerarin in Gelian Zhixie granule. Methods: The chromatographic conditions were Beckman ODS C 18 colum,mobile phrase of methonal -2.5% acetic acid (25∶75),flow rate of 1.0 ml·min -1 and detection wavelength at 250 nm. Results: The standard curve was linear over the range of 0.1～1 μg with the correlation coefficient 0.9998.The average recovery was 98.64% with RSD=1.72%(n=5). Conclusion: The HPLC method is sensitive, rapid and accurate. It can be used to control the quality of Gelian Zhixie granule.

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Available abstract

Objective: To establish a HPLC method for the determination of puerarin in Gelian Zhixie granule. Methods: The chromatographic conditions were Beckman ODS C 18 colum,mobile phrase of methonal -2.5% acetic acid (25∶75),flow rate of 1.0 ml·min -1 and detection wavelength at 250 nm. Results: The standard curve was linear over the range of 0.1～1 μg with the correlation coefficient 0.9998.The average recovery was 98.64% with RSD=1.72%(n=5). Conclusion: The HPLC method is sensitive, rapid and accurate. It can be used to control the quality of Gelian Zhixie granule.

Key concepts: Chromatography, Granule (geology), Puerarin, High-performance liquid chromatography, Chemistry, Correlation coefficient, Linear correlation, Linear range

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