ScholarLens Back to search
2007•Anhui Medical and Pharmaceutical JournalRequires access

Determination of puerarin in ganmaozhike granule by HPLC

HU An-qing

Open publisher page 0 citations

Abstract

Aim To determine the content of puerarin by RP-HPLC in Ganmaozhike granule.Methods The determination was carried out with Kromasil C18 column.The mobile phase consisted of CH3OH-1.8% CH3COOH(25∶ 75).The flow rate was 1ml·min-1 and the detection wavelength was at 250 nm.Results The content of ganmaozhike granule puerarin can be determined by HPLC.The linear concentration range was 5.915～47.32 μg and the average recovery was 99.4%,with RSD being 0.5%(n=6).Conclusion The method is simple,accurate and can be used to control the quality of ganmaozhike granule.

About this research paper

What this paper is about

Aim To determine the content of puerarin by RP-HPLC in Ganmaozhike granule.Methods The determination was carried out with Kromasil C18 column.The mobile phase consisted of CH3OH-1.8% CH3COOH(25∶ 75).The flow rate was 1ml·min-1 and the detection wavelength was at 250 nm.Results The content of ganmaozhike granule puerarin can be determined by HPLC.The linear concentration range was 5.915～47.32 μg and the average recovery was 99.4%,with RSD being 0.5%(n=6).Conclusion The method is simple,accurate and can be used to control the quality of ganmaozhike granule.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Aim To determine the content of puerarin by RP-HPLC in Ganmaozhike granule.Methods The determination was carried out with Kromasil C18 column.The mobile phase consisted of CH3OH-1.8% CH3COOH(25∶ 75).The flow rate was 1ml·min-1 and the detection wavelength was at 250 nm.Results The content of ganmaozhike granule puerarin can be determined by HPLC.The linear concentration range was 5.915～47.32 μg and the average recovery was 99.4%,with RSD being 0.5%(n=6).Conclusion The method is simple,accurate and can be used to control the quality of ganmaozhike granule.

Key concepts: Puerarin, Granule (geology), Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Content determination, Linear relationship

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of puerarin in ganmaozhike granule by HPLC — Research Paper | ScholarLens