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2006•Zhongguo yaofangRequires access

Content Determination of Puerarin in Qingshang Granule by HPLC

Ying Zeng

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Abstract

OBJECTIVE:To establish a method for content determination of puerarin in Qingshang Granule by HPLC. METHODS:A spherisorb C18 column was used;the mobile phase was methanol-acetic acid(25∶75);the flow rate was 1.2ml/min;the detecting wavelength was 250nm,and the column temperature was 45℃.RESULTS:The linear range of puerarin was within 10～100μg/ml(r=0.9 994).The average spotting recovery was 98.11%(RSD=0.32%).CONCLUSION:The established method is convenient,accurate,and replicable,which can be used for the quality control of this preparation.

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What this paper is about

OBJECTIVE:To establish a method for content determination of puerarin in Qingshang Granule by HPLC. METHODS:A spherisorb C18 column was used;the mobile phase was methanol-acetic acid(25∶75);the flow rate was 1.2ml/min;the detecting wavelength was 250nm,and the column temperature was 45℃.RESULTS:The linear range of puerarin was within 10～100μg/ml(r=0.9 994).The average spotting recovery was 98.11%(RSD=0.32%).CONCLUSION:The established method is convenient,accurate,and replicable,which can be used for the quality control of this preparation.

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Available abstract

OBJECTIVE:To establish a method for content determination of puerarin in Qingshang Granule by HPLC. METHODS:A spherisorb C18 column was used;the mobile phase was methanol-acetic acid(25∶75);the flow rate was 1.2ml/min;the detecting wavelength was 250nm,and the column temperature was 45℃.RESULTS:The linear range of puerarin was within 10～100μg/ml(r=0.9 994).The average spotting recovery was 98.11%(RSD=0.32%).CONCLUSION:The established method is convenient,accurate,and replicable,which can be used for the quality control of this preparation.

Key concepts: Puerarin, Chromatography, High-performance liquid chromatography, Granule (geology), Chemistry, Content determination, Linear range, Materials science

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