Effect of Resveratrol on proliferation and apoptosis of human cervical cancer cell Line SiHa

AIM: To explore the effect of resveratrol(Res) on the apoptosis of cervical cancer SiHa cells and its related mechanism.METHODS: SiHa cells were cultured and treated with different concentrations(0,12.5,25,50,100,200,300,400,600 μmol/L) of Res for 24,48 and 72 h.The growth inhibition rate of SiHa cells was detected by MTT method,and cell apoptosis rate was analyzed by flow cytometry(FCM) using annexinV/propidium iodide(PI) double staining.Morphologic changes of apoptotic cells were observed by fluorescence microscope and caspase-3 activity was assessed by colorimetric assay.RESULTS: Res inhibited the proliferation of SiHa cells in a time-and dose-dependent manner(P0.05).After Res treatment at 0,200 and 300 μmol/L for 48 h,the apoptosis rate of SiHa cells was 1.1%,8.9% and 11.1%,respectively,while the necrosis rate was 3.1%,15.0% and 14.0%,respectively.The cells showed characteristic apoptotic morphologic changes under fluorescence microscope.After 200 μmol/L Res treatment for 8 h,the activity of caspase-3 increased,reached the peak at the 24 h and then declined gradually.After SiHa cells were treated with 50,100 and 200 μmol/L Res for 24 h,the activity of caspase-3 was 1.92,2.51 and 4.53 folds as high as that in control group(P0.05).CONCLUSION: Res inhibits the proliferation and induces the apoptosis of SiHa cells in a time-and dose-dependent manner by increasing caspase-3 activity.