Effect of Extraction of Pelagi-Margarita on Proliferation and Apoptosis of Human Cervical Cancer Cell Line Siha

To explore the effect of extraction of pelagi-margarita(EPM)on the apoptosis of cervical cancer Siha cell line,the growth inhibition rate of Siha cells was detected by MTT method,and cell apoptosis rate was analyzed by flow cytometry(FCM)using annexinV/propidium iodide(PI)double staining,cell cycle was detected by flow cytometry using PI staining,morphologic changes of apoptotic cells were observed by fluorescence microscope,expression of intracellular Bcl-2,Bax protein was analyzed by real time PCR.The results showed that EPM inhibited the proliferation of Siha cells in a dose-dependent manner(P 0.05).After EPM treatment at 0,6,30 and 60 μg/mL for 24 h,the apoptosis rate of Siha cells was 5%,7.1%,32.25% and 31.95%,respectively,while the necrosis rate was 0.9%,1.55%,2.55%and 40.05%.The cells showed characteristic apoptotic morphologic changes under fluorescence microscope.Bioenergy vigor at various concentration could down-regulate the expression of Bcl-2 mRNA,meanwhile,up-regulate the expression of Bax mRNA.It concludes EPM inhibits the proliferation in a dose-dependent manner,and induces theapoptosis of Siha cells by down-regulate the expression of Bcl-2 mRNA,meanwhile,up-regulate the expression of Bax mRNA.