RhPDCD5 protein can enhance the apoptosis of SiHa ceivical cells induced by IFNγ

Objective To study the effect of rhPDCD5 protein on apoptosis of SiHa cervical cancer cells induced by IFNγ.Methods Human SiHa cervical cancer cells were treated with IFNγ 1×10～1×10 4 IU/ml prepared in serum-free DMEM. The cell proliferation was determined after 20,24,48,72 hours of incubation by MTT assay. Appropriate cell density and incubation time were selected for further study. The cells was treated with different concentrations of rhPDCD5 protein in the presence of IFNγ1×10 3 -1×10 4 IU/ml. Annexin V-labeled FACS assay were applied to study the apoptosis of SiHa cervical cancer cells. Results Incubated with IFNγ 1×10 3～1×10 4 IU/ml for 20 hours suppressed the proliferation of human SiHa cervical cancer cells significantly. The SiHa apoptotic cells were 5 67%,12 91% and 20 88%,increasing with the IFNγ of 1×10 2, 1×10 3 and 1×10 4 IU/ml. The apoptotic rote of SiHa cells that treated with 1×10 3IU/ml of IFNγplus 10 mg/L and 20 mg/L of rhPDCD5 protein were 24 74% and 26 89%, respectively. The apoptotic rate of SiHa cervical cancer cells treated with 1×10 4 IU/ml IFNγplus 10 mg/L and 20mg/L of rhPDCD5 protein were 32 69% and 32 27% respectively. Conclusions IFNγ could inhibits proliferation of SiHa cervical cancer cells by inducing apoptosis. RhPDCD5 protein enhances the apoptotic effect induced by IFNγ in SiHa cells.