Study on the resveratrol induced apoptosis in hunman cervical cancer cell line HeLa

Objective:To explore the effect of resveratrol on the apoptosis of cervical cancer HeLa cell.Methods:HeLa cells were cultured and treated with different concentrations(0,12.5,25,50,100,200,300,400,600μmol/L) of Res for 24,48,72h.The growth inhibition rate was detected by MTT method,and the cell apoptosis was analyzed with flow cytometry(FCM) using annexinV/propidium iodide(PI) double staining.Morphologic configuration of apoptotic cells were observed with fluorescence microscope,and caspase-3 activity was assessed by colorimetric assay.Results:Res inhibited the proliferation of HeLa cells in a time-and dosage-dependent manner(P0.05).After Res treatment at 0,200,300μmol/L for 48h,the apoptosis rates of HeLa cells were 2.2%,7.1%,6.23%,while the necrosis rates were 7.7%,16.04%,15.43%,respectively.The cells showed significant apoptotic morphological changes under fluorescence microscope.After 200μmol/L Res treatment for 8h,the activity of caspase-3 activity increased,and reached the peak at the 24h,then declined gradually.After HeLa cells were treated with 50,100,200μmol/L Res for 24h,the activity of caspase-3 was found increased by 1.92,2.51,4.53 folds compared with that of the control group(P0.05).Conclusion:Res can inhibit the proliferation and induce apoptosis of HeLa cells in a timeand dosage-dependent manner by increasing caspase-3 activity.