Expression of Toll-like Receptors 3,7,8 and 9 mRNA Expression in Different Tissues of Chinese Mongolian Horse

According to the established SYBR Green Ι fluorescent-based real-time reverse transcription polymerase chain reaction(RT-PCR) method for detection of horse toll-like receptor mRNA,the mRNA levels of TLR3,TLR7,TLR8 and TLR9 were analyzed in different tissues of Mongolia horse.The results showed the TLRs mRNA could be detected in heart,liver,spleen,lung,kidney,stomach,duodenum,jejunum,caecum and bone marrow.TLR3 mRNA level was the highest in kidney and the lowest in spleen.In contrast to TLR3,TLR7,TLR8 and TLR9 mRNA levels were the highest in spleen.TLR7 and TLR8 mRNA levels were the lowest in stomach,TLR9 was lowest in jejunum.In different immune organs,TLR7,TLR8 and TLR9 mRNA levels were higher in spleen than that in bone marrow,while the expression of TLR3 mRNA was opposite.In different intestinal segments,TLR3,TLR7,TLR8 and TLR9 mRNA levels were higher in caecum than in duodenum and jejunum.The expression of TLR3 and TLR8 mRNA were higher than TLR7 and TLR9 mRNA.The results indicated that there was comparatively large difference on the transcription level of TLRs mRNA in different tissues,which may be associated with the ability to recognize pathogens and different resistance in Mongolia horse.