2015•Heilongjiang xumu shouyiRequires access
Research on TLR3,TLR7,TLR8 and TLR9 mRNA expression levels in different tissues from the horses with food poisoning
Zhao Yi-pin
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Abstract
To investigate the expression levels of TLR3,TLR7,TLR8 and TLR9 genes in different tissues and organs from the horses with food poisoning,SYBR Green Ⅰ- based fluorescence quantitative RT- PCR assay was used to detect the transcription levels of TLRs in the stomach,heart,liver,spleen,lung,kidney,duodenum,cecum,and bone marrow from the horses with food poisoning and healthy horses as a control group. The results showed that the relative expression levels of TLR3,TLR7,TLR8,TLR9 genes in the stomach,liver and duodenum of the horses with food poisoning were significantly higher( P 0. 05) than those from the control group. The differences in the expression levels of TLR9 gene in the 9 tissues and organs except lung from the horses detected with food poisoning were significant( P 0. 05) compared with health control group. The results indicate that food poisoning can cause changes in the stomach,liver and duodenum of horses,it is guessed that TLR9 gene plays an important role in the body against food poisoning.