mRNA expression of Toll-like receptors 1,2,4 and 6 in different tissues of Mongolia horse

According to the sequences of horse Toll-like receptor(TLR) in GenBank,the real-time PCR available primers were designed and synthesized to establish a method for the detection of mRNA transcription of TLRs by real-time quantitative PCR and analyze the transcription of TLR4,TLR2,TLR1 and TLR6 in different tissues of Mongolia horse.The results showed the TLRs mRNA was transcripted in heart,liver,spleen,lung,kidney,stomach,duodenum,jejunum,caecum and medulla.The level of TLR4 mRNA was higher than TLR2,TLR1 and TLR6 mRNA in all of above-mentioned tissues except jejunum and liver.In immune organs,TLR4 and TLR1 mRNA level was higher in spleen than in medulla,but TLR2 and TLR6 mRNA level was higher in medulla than in spleen.In different instestinal part,TLR4,TLR2,TLR1 and TLR6 mRNA level were little difference in jejunum,but had large difference in duodenum and caecum.The result indicated that there was comparatively large difference in the transcription level of TLRs mRNA in different tissues,which may be associated with the ability to recognize pathogens and different resistance in horse.