Comparison and Determination of Total Flavonoids in Different Parts of Berchemia lineata

Objective: The content distribution of total flavonoids in different parts of Berchemia lineate from city of DuYun in Guizhou was investigated.Method:Flavonids in different parts of Berchemia Lineate(L)DC.were extracted by ethanol as solvent reflux,The total flavonoids were determined by spectrophotometry,rutin as control sample.Result:The absorbency and concentration of rutin have good linear correlation in the range of 5-30 μg·L-1.The regression equation was Y=0.011 2X+0.041 9(r=0.999 1),the average recovery rate was 98.56%,Accuracy experiment RSD 0.279%,repeatability test RSD 0.634%,stability test RSD 1.49%,the contents of total flavonoids in leaf and stem was respectively 12.30% and 1.42%,that the means content of total flavonoids in leaf is 8.7 times higher than that in stem.Conclusion: the content of total flavonoids in the leaf and in the stem has a big difference,thereby the leaf of B.lineate from Duyun in Guizhou has value to be developed and used.