ScholarLens Back to search
2010•Shandong yiyaoRequires access

Experimental study of inhibitory effects of silver nanoparticles on influenza virus H3N2

Zheng Cong-long

Open publisher page 1 citations

Abstract

Objective To study the inhibitory effects of silver nanoparticles on influenza virus H3N2.Methods MTT test,cytopathic effect(CPE) test,transmission electron microscopy(TEM) and flow cytometry were used to determine the inhibitory effects of silver nanoparticles on influenza virus H3N2.Results The maximal atoxic concentration(TD0) of silver nanoparticles and the tissue culture infective dose(TCID50) of H3N2 on MD-25 cell was 25 μg/ml and 10-3.5/0.1 ml,respectively.The apoptosis rate of MD-25 cell was(5.51±1.06)% after the 50 μg/ml nanosilver solution mixed with 40 TCID50 influenza virus H3N2 for 2 hours and the CPE was negative,the apoptosis rate of MD-25 cell in 20 TCID50 influenza virus H3N2 group was(22.54±2.57)% and the CPE was positive(P0.01).Conclusion Nanosilver has obvious inhibitory effects on influenza virus H3N2.

About this research paper

What this paper is about

Objective To study the inhibitory effects of silver nanoparticles on influenza virus H3N2.Methods MTT test,cytopathic effect(CPE) test,transmission electron microscopy(TEM) and flow cytometry were used to determine the inhibitory effects of silver nanoparticles on influenza virus H3N2.Results The maximal atoxic concentration(TD0) of silver nanoparticles and the tissue culture infective dose(TCID50) of H3N2 on MD-25 cell was 25 μg/ml and 10-3.5/0.1 ml,respectively.The apoptosis rate of MD-25 cell was(5.51±1.06)% after the 50 μg/ml nanosilver solution mixed with 40 TCID50 influenza virus H3N2 for 2 hours and the CPE was negative,the apoptosis rate of MD-25 cell in 20 TCID50 influenza virus H3N2 group was(22.54±2.57)% and the CPE was positive(P0.01).Conclusion Nanosilver has obvious inhibitory effects on influenza virus H3N2.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To study the inhibitory effects of silver nanoparticles on influenza virus H3N2.Methods MTT test,cytopathic effect(CPE) test,transmission electron microscopy(TEM) and flow cytometry were used to determine the inhibitory effects of silver nanoparticles on influenza virus H3N2.Results The maximal atoxic concentration(TD0) of silver nanoparticles and the tissue culture infective dose(TCID50) of H3N2 on MD-25 cell was 25 μg/ml and 10-3.5/0.1 ml,respectively.The apoptosis rate of MD-25 cell was(5.51±1.06)% after the 50 μg/ml nanosilver solution mixed with 40 TCID50 influenza virus H3N2 for 2 hours and the CPE was negative,the apoptosis rate of MD-25 cell in 20 TCID50 influenza virus H3N2 group was(22.54±2.57)% and the CPE was positive(P0.01).Conclusion Nanosilver has obvious inhibitory effects on influenza virus H3N2.

Key concepts: Virus, Apoptosis, Flow cytometry, Inhibitory postsynaptic potential, Influenza A virus, Cytopathic effect, Chemistry, Silver nanoparticle

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Experimental study of inhibitory effects of silver nanoparticles on influenza virus H3N2 — Research Paper | ScholarLens