Inactivation of nanosilver on influenza virus H3N2

[Objective]To study the inactivation of nanosilver(NaAg) on influenza virus H3N2.[Methods]The hemagglutination test,the cultivation in the chick embryo test,the MTT test and the haemocyanin Absorption test were used to determine the inactivation of NaAg on influenza virus H3N2.The TEM and the flow cytometer were adopted to detected the apoptosis of cells induced by virus.[Results]In NaAg/H3N2 group,the titer of hemagglutination and that of allantoic fluid of chick embryo was 1∶2 and lower than 1∶2,but in the control group of influenza virus,the titers of both were 1∶1024(P0.001).The maximal atoxic concentration(TD0) of NaAg on MDCK cell was 25 μg/mL,the TCID50 of influenza virus H3N2 on MDCK cell was 10-3.5/0.1 mL.The survival rate of MDCK cells was(98.16±2.67)% after the 50 μg/mL NaAg solution mixed with 40 TCID50 of influenza virus H3N2 in 2 hours,and the survival rate of MDCK cells was(35.21±1.45)% in the influenza virus H3N2 control group with 20 TCID50(P0.001).NaAg of 25 μg/mL inhibited effectively the apoptosis of MDCK cells induced by 20 TCID50 influenza virus H3N2.[Conclusion]The nanosilver could inactivatInfluenza virus H3N2.