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Determination of Paeoniflorin in Erlongzuoci Pills by HPLC

Zhu Shan-yin

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Abstract

Objective To establish the method for determining the content of paeoniflorin in Erlongzuoci Pills by HPLC.Methods The Suntek C 18 column with acetonitrile-water(30∶70 ) as the mobile phase was used.The flow rate was 1.0 mL/min and the detective wavelength was 230 nm.Result The calibration curve was linear at a range of 20~120 μg/mL for paeoniflorin(r=0.999 4).The average recovery was 99.79% and RSD was 1.52% (n=6).Conclusion The method is simple,and accurate with a good reproducibility and can be used as quantitative analysis method for this preparation.

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What this paper is about

Objective To establish the method for determining the content of paeoniflorin in Erlongzuoci Pills by HPLC.Methods The Suntek C 18 column with acetonitrile-water(30∶70 ) as the mobile phase was used.The flow rate was 1.0 mL/min and the detective wavelength was 230 nm.Result The calibration curve was linear at a range of 20~120 μg/mL for paeoniflorin(r=0.999 4).The average recovery was 99.79% and RSD was 1.52% (n=6).Conclusion The method is simple,and accurate with a good reproducibility and can be used as quantitative analysis method for this preparation.

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Available abstract

Objective To establish the method for determining the content of paeoniflorin in Erlongzuoci Pills by HPLC.Methods The Suntek C 18 column with acetonitrile-water(30∶70 ) as the mobile phase was used.The flow rate was 1.0 mL/min and the detective wavelength was 230 nm.Result The calibration curve was linear at a range of 20~120 μg/mL for paeoniflorin(r=0.999 4).The average recovery was 99.79% and RSD was 1.52% (n=6).Conclusion The method is simple,and accurate with a good reproducibility and can be used as quantitative analysis method for this preparation.

Key concepts: Paeoniflorin, Chromatography, Calibration curve, High-performance liquid chromatography, Reproducibility, Chemistry, Linear range, Analytical Chemistry (journal)

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