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2007•Strait Pharmaceutical JournalRequires access

Determination of paeoniflorin in Baidai pills by HPLC

Yang Min-cong

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Abstract

OBJECTIVE To establish a HPLC method for the determination of the contentof Paeoniflorin in Baidai Pills.METHODS Kromasil 100-5C18(250mm×4.6mm)column was used,the mobile phase was methanol,rate was set at 1.0mL·min-1,the detection wave length was 230nm;and column temperature was room temperature.RESULTS The calibration curve was linear(r=0.9999,n=5)in the range of 0.9224～2.244μg for Paeoniflorin,the average recovery was 99.84% RSD was 0.34%(n=9).CONCLUSION This method may be accurate,sample sensitive and reliable and it can be ed as the quality control of Baidai pills.

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What this paper is about

OBJECTIVE To establish a HPLC method for the determination of the contentof Paeoniflorin in Baidai Pills.METHODS Kromasil 100-5C18(250mm×4.6mm)column was used,the mobile phase was methanol,rate was set at 1.0mL·min-1,the detection wave length was 230nm;and column temperature was room temperature.RESULTS The calibration curve was linear(r=0.9999,n=5)in the range of 0.9224～2.244μg for Paeoniflorin,the average recovery was 99.84% RSD was 0.34%(n=9).CONCLUSION This method may be accurate,sample sensitive and reliable and it can be ed as the quality control of Baidai pills.

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Available abstract

OBJECTIVE To establish a HPLC method for the determination of the contentof Paeoniflorin in Baidai Pills.METHODS Kromasil 100-5C18(250mm×4.6mm)column was used,the mobile phase was methanol,rate was set at 1.0mL·min-1,the detection wave length was 230nm;and column temperature was room temperature.RESULTS The calibration curve was linear(r=0.9999,n=5)in the range of 0.9224～2.244μg for Paeoniflorin,the average recovery was 99.84% RSD was 0.34%(n=9).CONCLUSION This method may be accurate,sample sensitive and reliable and it can be ed as the quality control of Baidai pills.

Key concepts: Paeoniflorin, Chromatography, Calibration curve, High-performance liquid chromatography, Chemistry, Analytical Chemistry (journal), Detection limit

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