2010•China Medical HeraldRequires access

Content determination of paeoniflorin in Yaoxitong Pills by HPLC

Anping Yang

Abstract

Objective:To establish a method for the content determination of paeoniflorin in Yaoxitong Pills by HPLC. Methods:The Thermo ODS-2 Hypersil (4.6 mm×150 mm,5 μm) column was used. The mobile phases consisted of acetonitrile and 0.1%-phosphoric acid (13∶87),the flow rate was 1.0 ml/min and the detection wavelength was at 230 nm. Results:The linear ranges of paeoniflorin was at 0.04-0.88 μg (r=0.999 9),the average recovery was 101.50% with a RSD of 1.20%. Conclusion:The method is fast,reliable,accurate and can be used in the quality control of paeoniflorin in Yaoxitong Pills.

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Key concepts: Paeoniflorin, Medicine, Chromatography, Phosphoric acid, High-performance liquid chromatography, Content determination, Pill, Pharmacology