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Determination of Paeoniflorin in Tiaojinghuoxue Pills by HPLC

Zhou Song

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Abstract

Objective To establish a HPLC method for determining the content of paeoniflorin in Tiaojinghuoxue pills. Methods A Kromasil ODS C18 column (250 mm×4.6 mm, 5μm) was used. The mobile phase was isopropanol-methanol-water (1:23:76) at a flow rate of 1.0 ml/min. The detection wavelength was 230 nm. Results The linear range of paeoniflorin was 0.221～1.326 μg/ml (r=1.0000).The average recovery (n=6) was 100. 42%. RSD was 0.55%. Conclusion The method is simple, reliable, accurate, and it can be applied to the quality control of the preparation.

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What this paper is about

Objective To establish a HPLC method for determining the content of paeoniflorin in Tiaojinghuoxue pills. Methods A Kromasil ODS C18 column (250 mm×4.6 mm, 5μm) was used. The mobile phase was isopropanol-methanol-water (1:23:76) at a flow rate of 1.0 ml/min. The detection wavelength was 230 nm. Results The linear range of paeoniflorin was 0.221～1.326 μg/ml (r=1.0000).The average recovery (n=6) was 100. 42%. RSD was 0.55%. Conclusion The method is simple, reliable, accurate, and it can be applied to the quality control of the preparation.

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Available abstract

Objective To establish a HPLC method for determining the content of paeoniflorin in Tiaojinghuoxue pills. Methods A Kromasil ODS C18 column (250 mm×4.6 mm, 5μm) was used. The mobile phase was isopropanol-methanol-water (1:23:76) at a flow rate of 1.0 ml/min. The detection wavelength was 230 nm. Results The linear range of paeoniflorin was 0.221～1.326 μg/ml (r=1.0000).The average recovery (n=6) was 100. 42%. RSD was 0.55%. Conclusion The method is simple, reliable, accurate, and it can be applied to the quality control of the preparation.

Key concepts: Paeoniflorin, Chromatography, High-performance liquid chromatography, Content determination, Linear range, Chemistry, Detection limit

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