The clinical study of NF-κB in diabetics with the different level of urinary albumin

Objective Objective To approach the significance of nuclear factor-kappaB(NF-κB) in diabetic nephropathy by comparing the levels of NF-κB which expressed on peripheral blood mononuclear cells in diabetics with the different level of urinary albumin.Methods Total 20 normal controls and 50 type 2 diabetics were recruited into the study.The latter were divided into 3 groups according to the urinary albumin excretion rate(UAER):①normoalbuminuria group(UAER20 μg/min,groupA=25);②microalbuminuria group(20 μg/min≤UAER200 μg/min,group B=15);③macro-albuminuria group(UAER≥200 μg/min,groupC=10).NF-κB were measured by enzyme linked immunosorbent assay(ELISA).Results The expressions of NF-κB on peripheral blood mononuclear cells in T2DM were significantly higher than those in the controls(P0.01),The levels in the group B were obviously higher than those in the group A(P0.05) and increased further in the group C(P0.05).likewise,the group C was higher than the group B(P0.05).The relative analysis showed that the NF-κB were positively related to UAER,serum creatinine,the course of disease and HbA1c(r=0.715,r=0.701,r=0.514,P all0.01;r=0.308,P0.05).Conclusion Increased expressions of NF-κB might take a important part in the progression of diabetic nephropathy.