2013•Zhongguo shiyan fangjixue zazhiRequires access
Optimization of Purification Process for Total Flavonoids from Baeckea frutescens L.
Qiu Hong-cong
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Abstract
Objective: To optimize purification process of total flavonoids from Baeckea frutescens L.by macroporous adsorption resin.Method: Different types of resin were selected with absorption and desorption ratio of total flavonoids as indexes,the content of total flavonoids was determined by UV,single factor test was adopted to investigate purification technology of total flavonoids by macroporous adsorption resin.Result: AB-8 type resin was adopted,its optimum process conditions were as followings: the concentration of sample solution 0.3 g.mL-1,absorption velocity 1 BV.h-1,sample volume 3 BV,eluted by 5 BV 50% ethanol at flow rate of 2 BV.h-1.Under these conditions,purity of total flavonoids was up to 52.33%.Conclusion: Optimized process was rational,feasible and suitable for industrial production.