Extraction and Purification Technology of Total Flavonoids for Scutellaria baicalensis and Sedum sarmentosum from Longchaifang

Objective:To optimize extraction and purification technology of total flavonoids for S.baicalensis and S.sarmentosum from Longchaifang.Method:The content of total flavonoids were selected as index,extraction and purification technology condition of total flavonoids was optimized by orthogonal test.Total flavonoids was purified by macroporous resin,screened optimum purification macroporous resin by static adsorption and desorption test;Optimum elution condition was examined by related indicators of dynamic adsorption.Result:Optimum extraction process was as follows:reflux extracted 2 times with 15 times the anount of 60% ethanol at 85 ℃,2 h each time.Optimum purification technology was as follows:AB-8 macroporous resin was adopted,concentration of sample was 0.11 g·mL-1,adsorptive speed was 3 BV·h-1,adsorbing column was eluted with 5 BV waterand 8 BV 70% ethanol.Conclusion:The content of the total flavonoids was high by optimization technology.This technique was simple and suitable for modern production.