Immunodiagnostic value of rSj26-Sj32-HRP-IgG-Dot-ELISA for patients with acute schistosomiasis japonica.
Min Wang, Wengui Li, Cai ShiFei, Biying Zhou
Abstract
Min Wang, Wengui Li, Cai ShiFei, Biying Zhou
Abstract
To study the immunodiagnostic value of rSj26-Sj32-HR-IgG-Dot-ELISA for atients with acute Schistosomiasis jaonica,sera from arients were detected by HR-IgG-Dot-ELISA which established by urified rSj26-Sj32 fusion rotein and Schistosoma jaonicum adult worm antigen(SjAWA),and comared with detections results of sera from atients with clonorchiasis,aragonimiasis westermani,echinococciasis,heatitis B and ulmonary tuberculosis,as well as healthy eole.It showed that both of the ositive rates for rSj26-Sj32 and SjAWA in detection of sera from atients with acute schistosomiasis jaonica were 100%,and the secificities were 95.35% and 93.02%,resectively.Moreover,cross reactions different degrees were emerged between sera from atients with clonorchiasis,aragonimiasis westermani and alveolar echinococcosis and both of rSj26-Sj32 and SjAWA.It is suggested that the urified rSj26-Sj32 fusion ortein is one of the referable immunodiagnostic antigen for schistosomiasis jaonica.
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To study the immunodiagnostic value of rSj26-Sj32-HR-IgG-Dot-ELISA for atients with acute Schistosomiasis jaonica,sera from arients were detected by HR-IgG-Dot-ELISA which established by urified rSj26-Sj32 fusion rotein and Schistosoma jaonicum adult worm antigen(SjAWA),and comared with detections results of sera from atients with clonorchiasis,aragonimiasis westermani,echinococciasis,heatitis B and ulmonary tuberculosis,as well as healthy eole.It showed that both of the ositive rates for rSj26-Sj32 and SjAWA in detection of sera from atients with acute schistosomiasis jaonica were 100%,and the secificities were 95.35% and 93.02%,resectively.Moreover,cross reactions different degrees were emerged between sera from atients with clonorchiasis,aragonimiasis westermani and alveolar echinococcosis and both of rSj26-Sj32 and SjAWA.It is suggested that the urified rSj26-Sj32 fusion ortein is one of the referable immunodiagnostic antigen for schistosomiasis jaonica.
Key concepts: Clonorchiasis, Schistosomiasis, SCHISTOSOMIASIS JAPONICA, Schistosoma japonicum, Medicine, Paragonimiasis, Immunology, Antigen