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2010•Journal of Tropical MedicineOpen access

Diagnostic value of rSj26-Sj32-immunogold-IgG-Dot-ELISA assay in the diagnosis of acute schistosomiasis japonicum.

Min Wang, Wengui Li, Cai ShiFei, Biying Zhou

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Abstract

Objective To study the diagnostic value of rSj26-Sj32 fusion protein-Immunogold-IgG-Dot-ELISA in the diagnosis of acute schistosomiasis japonicum.Methods Purified rSj26-Sj32 protein and Schistosoma japonicum adult worm antigen(SjAWA) were used in the Immunogold-IgG-Dot-ELISA.Sera from patients with acute schistosomiasis japonicum were used in this analysis.Control sera were obtained from patients with paragonimiasis,clonorchiasis,echinococciasis,hepatitis B,lung tuberculosis and the normal subjects.Results All patients with acute schistosomiasis japonicum were positively diagnosed using rSj26-Sj32 fusion protein and SjAWA antigens.The specificity for the testing using rSj26-Sj32 fusion protein and SjAWA was 97.67% and 95.35%,respectively.Cross-reaction was observed in the sera from patients with paragonimiasis and clonorchiasis when SjAWA was used.Conclusion Recombinant Sj26-Sj32 may be used for the diagnosis of schistosomiasis.

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Objective To study the diagnostic value of rSj26-Sj32 fusion protein-Immunogold-IgG-Dot-ELISA in the diagnosis of acute schistosomiasis japonicum.Methods Purified rSj26-Sj32 protein and Schistosoma japonicum adult worm antigen(SjAWA) were used in the Immunogold-IgG-Dot-ELISA.Sera from patients with acute schistosomiasis japonicum were used in this analysis.Control sera were obtained from patients with paragonimiasis,clonorchiasis,echinococciasis,hepatitis B,lung tuberculosis and the normal subjects.Results All patients with acute schistosomiasis japonicum were positively diagnosed using rSj26-Sj32 fusion protein and SjAWA antigens.The specificity for the testing using rSj26-Sj32 fusion protein and SjAWA was 97.67% and 95.35%,respectively.Cross-reaction was observed in the sera from patients with paragonimiasis and clonorchiasis when SjAWA was used.Conclusion Recombinant Sj26-Sj32 may be used for the diagnosis of schistosomiasis.

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Available abstract

Objective To study the diagnostic value of rSj26-Sj32 fusion protein-Immunogold-IgG-Dot-ELISA in the diagnosis of acute schistosomiasis japonicum.Methods Purified rSj26-Sj32 protein and Schistosoma japonicum adult worm antigen(SjAWA) were used in the Immunogold-IgG-Dot-ELISA.Sera from patients with acute schistosomiasis japonicum were used in this analysis.Control sera were obtained from patients with paragonimiasis,clonorchiasis,echinococciasis,hepatitis B,lung tuberculosis and the normal subjects.Results All patients with acute schistosomiasis japonicum were positively diagnosed using rSj26-Sj32 fusion protein and SjAWA antigens.The specificity for the testing using rSj26-Sj32 fusion protein and SjAWA was 97.67% and 95.35%,respectively.Cross-reaction was observed in the sera from patients with paragonimiasis and clonorchiasis when SjAWA was used.Conclusion Recombinant Sj26-Sj32 may be used for the diagnosis of schistosomiasis.

Key concepts: Clonorchiasis, Schistosoma japonicum, Schistosomiasis, Paragonimiasis, Immunogold labelling, Immunology, Antigen, Paragonimus

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Diagnostic value of rSj26-Sj32-immunogold-IgG-Dot-ELISA assay in the diagnosis of acute schistosomiasis japonicum. — Research Paper | ScholarLens