2010•Journal of Pathogen BiologyRequires access
Study on the diagnosis of chronic schistosomiasis japonicum using rSj26-Sj32-immunogold-IgG-dot-ELISA.
Cai ShiFei, Wengui Li, Wang Min, Biying Zhou
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Abstract
Objective To study the value of rSj26-Sj32-Immunogold-IgG-Dot-ELISA in the diagnosis of chronic schistosomiasis japonicum.Methods IgG antibodies were detected in sera from patients with chronic schistosomiasis japonicum using rSj26-Sj32 fusion protein and SjAWA in immunogold-IgG-Dot-ELISA.The sera of patients with clonorchiasis,paragonimiasis westermani,alveolar echinococcosis,cystic echinococcosis,hepatitis B,or pulmonary tuberculosis and the sera of healthy people served as a control.Results rSj26-Sj32-immunogold-IgG-dot-ELISA had a sensitivity and specificity of 95.00% and 97.67%,respectively,and there was no cross-reaction with the sera from patients with clonorchiasis,paragonimiasis westermani,alveolar echinococcosis,cystic echinococcosis,hepatitis B,or pulmonary tuberculosis.The technique had a positive predictive value and negative predictive value of 97.44% and 95.45%,respectively,and its diagnostic efficiency was 96.39%,the same as that of SjAWA-Immunogold-IgG.Conclusion rSj26-Sj32-immunogold-IgG-dot-ELISA may be used in the immunodiagnosis of chronic schistosomiasis japonicum.