2007•The Chinese Journal of Modern Applied PharmacyRequires access
Study on the Determination of Azithromycin Content by Spectrophotometry Reaction
Zhao Gui-zhi
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Abstract
OBJECTIVE To determine the content of azithromycin through the reaction between azithromycin and xylenol orange.METHODS Utilizing the color reaction between azithromycin and xylenol orange in the ethanol-water solution,a spectrophotometic method was used to the determination of Azithromycin content. RESULTS The color reaction between azithromycin and xylenol orange in ethanol-water could form the complex of azithromycin and xylenol orange.The apparent molar absorptivity of complex at 582 nm is 1.04×104L·mol-1·cm-1,stability constant is 5.06×105,the composition of the complex between azithromycin and xylenol orange was 2∶1.The average standard deviation is 0.96%(n=8).Beer's law is obeyed in the range of 10~70 mg·L-1 of azithromycin in tablets.CONCLUSION The method is simple with easily-controlled reaction conditions and high sensitivity.