Determination of Azithromycin by Modified Spectrophotometry

The spectrophotometric method for determination of azithromycin based on the color reaction of sulfuric acid and azithromycin was optimized and all variables reaction conditions such as sulfuric acid concentration,heating time,temperature were carefully studied.The result showed the maximum absorbance at 482nm is linear to concentration of azithromycin with A=0.0172C(mg·L-1)+0.0504 in the range of 11.26—45.03mg·L-1.The average recovery was 99.1%(n=9).This optimized method is simple,sensitive,accurate,and suitable for the rapid determination of azithromycin.