2005•Chinese Journal of PharmaceuticalsRequires access

Determination of Azithromycin Tablets by Spectrophotometry Based on Charge-transfer Reaction with Alizarin Red

Hong Shu Liu

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Abstract

A spectrophotometric method based on the charge-transfer reaction between azithromycin and alizarin redin aqueous ethanolic solution was established for the determination of azithromycin tablets. The composition ratio of thecomplex was 1∶2, and the apparent molar absorptivity was 1.23×104 L·mol-1·cm-1 at maximum absorption wavelengthof 538nm. The calibration curve was linear in the range of 10 - 60μg/ml. The results were comparable with those by HPLC.

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A spectrophotometric method based on the charge-transfer reaction between azithromycin and alizarin redin aqueous ethanolic solution was established for the determination of azithromycin tablets. The composition ratio of thecomplex was 1∶2, and the apparent molar absorptivity was 1.23×104 L·mol-1·cm-1 at maximum absorption wavelengthof 538nm. The calibration curve was linear in the range of 10 - 60μg/ml. The results were comparable with those by HPLC.

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Available abstract

A spectrophotometric method based on the charge-transfer reaction between azithromycin and alizarin redin aqueous ethanolic solution was established for the determination of azithromycin tablets. The composition ratio of thecomplex was 1∶2, and the apparent molar absorptivity was 1.23×104 L·mol-1·cm-1 at maximum absorption wavelengthof 538nm. The calibration curve was linear in the range of 10 - 60μg/ml. The results were comparable with those by HPLC.

Key concepts: Chemistry, Molar absorptivity, ALIZARIN RED, Chromatography, Spectrophotometry, Calibration curve, Alizarin, Aqueous solution

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