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2015•Pharmacy TodayRequires access

Determination of Paeoniflorin in Toutongling Capsules by HPLC

Xie Jian-min

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Abstract

OBJECTIVE To establish a method for the determination of paeoniflorin in Toutongling capsules by HPLC.METHODS HPLC was performed on a Venusil XBP C18column( 250 mm × 4. 6 mm,5 μm). The mobile phase was acetonitrile-0.05% phosphate( 14 ∶ 86),and flow rate was 1. 0 m L/min,column temperature 25 ℃. The detection wavelength was 230 nm.RESULTS The paeoniflorin had a good linearity in the ranges of 0.172 34- 1.723 4 μg( r = 1). The average recovery was 101.8%with RSD = 0.9%.CONCLUSION The method is for the determination of easy operation,reliable results,and reproducible. It can be used for the quality control of paeoniflorin in Toutongling capsules.

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What this paper is about

OBJECTIVE To establish a method for the determination of paeoniflorin in Toutongling capsules by HPLC.METHODS HPLC was performed on a Venusil XBP C18column( 250 mm × 4. 6 mm,5 μm). The mobile phase was acetonitrile-0.05% phosphate( 14 ∶ 86),and flow rate was 1. 0 m L/min,column temperature 25 ℃. The detection wavelength was 230 nm.RESULTS The paeoniflorin had a good linearity in the ranges of 0.172 34- 1.723 4 μg( r = 1). The average recovery was 101.8%with RSD = 0.9%.CONCLUSION The method is for the determination of easy operation,reliable results,and reproducible. It can be used for the quality control of paeoniflorin in Toutongling capsules.

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Available abstract

OBJECTIVE To establish a method for the determination of paeoniflorin in Toutongling capsules by HPLC.METHODS HPLC was performed on a Venusil XBP C18column( 250 mm × 4. 6 mm,5 μm). The mobile phase was acetonitrile-0.05% phosphate( 14 ∶ 86),and flow rate was 1. 0 m L/min,column temperature 25 ℃. The detection wavelength was 230 nm.RESULTS The paeoniflorin had a good linearity in the ranges of 0.172 34- 1.723 4 μg( r = 1). The average recovery was 101.8%with RSD = 0.9%.CONCLUSION The method is for the determination of easy operation,reliable results,and reproducible. It can be used for the quality control of paeoniflorin in Toutongling capsules.

Key concepts: Paeoniflorin, Chromatography, High-performance liquid chromatography, Chemistry, Acetonitrile, Phosphate buffered saline

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