ScholarLens Back to search
2009•Qilu Pharmaceutical AffairsRequires access

Determination of paeoniflorin in Bushentiaojing Capsules by HPLC

Ding Shao-chun

Open publisher page 0 citations

Abstract

OBJECTIVE To establish an HPLC method for determination of paeoniflorin in Bushentiaojing Capsules. METHODS The HPLC method was carried on C18 column using Acetonitrile-0.1% Phosphoric acid (15:85) as the mobile phase,and the detection wavelength was 230nm,the flow rate was 1.0mL· min~(-1) and the temperature of column was 35℃.RESULTS In the HPLC method,the calibration curve for paeoniflorin was linear in the range of 0.1024～1. 5360μg(r= 0. 9998) ,the average recovery was 99. 7% , RSD was 0.33%(n=5). CONCLUSION The method was convenient,precise and reliable for determining the content of paeoniflorin in Bushentiaojing Capsules.

About this research paper

What this paper is about

OBJECTIVE To establish an HPLC method for determination of paeoniflorin in Bushentiaojing Capsules. METHODS The HPLC method was carried on C18 column using Acetonitrile-0.1% Phosphoric acid (15:85) as the mobile phase,and the detection wavelength was 230nm,the flow rate was 1.0mL· min~(-1) and the temperature of column was 35℃.RESULTS In the HPLC method,the calibration curve for paeoniflorin was linear in the range of 0.1024～1. 5360μg(r= 0. 9998) ,the average recovery was 99. 7% , RSD was 0.33%(n=5). CONCLUSION The method was convenient,precise and reliable for determining the content of paeoniflorin in Bushentiaojing Capsules.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE To establish an HPLC method for determination of paeoniflorin in Bushentiaojing Capsules. METHODS The HPLC method was carried on C18 column using Acetonitrile-0.1% Phosphoric acid (15:85) as the mobile phase,and the detection wavelength was 230nm,the flow rate was 1.0mL· min~(-1) and the temperature of column was 35℃.RESULTS In the HPLC method,the calibration curve for paeoniflorin was linear in the range of 0.1024～1. 5360μg(r= 0. 9998) ,the average recovery was 99. 7% , RSD was 0.33%(n=5). CONCLUSION The method was convenient,precise and reliable for determining the content of paeoniflorin in Bushentiaojing Capsules.

Key concepts: Paeoniflorin, Chromatography, High-performance liquid chromatography, Calibration curve, Phosphoric acid, Chemistry, Linear range, Detection limit

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of paeoniflorin in Bushentiaojing Capsules by HPLC — Research Paper | ScholarLens