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2008•Strait Pharmaceutical JournalRequires access

Determination of paeoniflorin in Hanrebi capsules by HPLC

Zheng Yan-qing

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Abstract

OBJECTIVE To establish a method for determination of paeoniflorin in Hanrebi capsules.METHODS Using a HPLC method with Lichrospher ODS(4.6×250mm,5μm) column,the mobile phase was acetonitrile-0.1% phosphoric acid (13∶87),the detection wavelength was set at 230nm,flow rate was 1.0 mL·min-1,and the column temperature was 30℃.RESULTS The precision and stability were fine,the standard curve of paeoniflorin was rectilinear within the range of 0.137～0.684μg,r=0.9997.The average recovery of paeoniflorin was 99.80% with RSD of 1.43%.CONCLUSION The method was simple,accurate and reproducible,and could be used for the determination.

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What this paper is about

OBJECTIVE To establish a method for determination of paeoniflorin in Hanrebi capsules.METHODS Using a HPLC method with Lichrospher ODS(4.6×250mm,5μm) column,the mobile phase was acetonitrile-0.1% phosphoric acid (13∶87),the detection wavelength was set at 230nm,flow rate was 1.0 mL·min-1,and the column temperature was 30℃.RESULTS The precision and stability were fine,the standard curve of paeoniflorin was rectilinear within the range of 0.137～0.684μg,r=0.9997.The average recovery of paeoniflorin was 99.80% with RSD of 1.43%.CONCLUSION The method was simple,accurate and reproducible,and could be used for the determination.

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Available abstract

OBJECTIVE To establish a method for determination of paeoniflorin in Hanrebi capsules.METHODS Using a HPLC method with Lichrospher ODS(4.6×250mm,5μm) column,the mobile phase was acetonitrile-0.1% phosphoric acid (13∶87),the detection wavelength was set at 230nm,flow rate was 1.0 mL·min-1,and the column temperature was 30℃.RESULTS The precision and stability were fine,the standard curve of paeoniflorin was rectilinear within the range of 0.137～0.684μg,r=0.9997.The average recovery of paeoniflorin was 99.80% with RSD of 1.43%.CONCLUSION The method was simple,accurate and reproducible,and could be used for the determination.

Key concepts: Paeoniflorin, Chromatography, Phosphoric acid, Chemistry, High-performance liquid chromatography, Acetonitrile, Organic chemistry

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