Determination of Paeoniflorin in Jiangyaning Capsules by HPLC

Objective To establish HPLC method for the determination of paeoniflorin in Jiangyaning capsules.Methods The determination was performed on Kromasil C18(5 μm,150 nm×4.6 nm),the mobile phase consisted of acetonitrile-water-acetic acid(28:72:0.5)with a flow rate of 1.0 ml/min.The column temperature was 30℃;the detecation wavelength was 230nm and the sample size was 10 μl.Results Good linear relationship with peak area occurred when the sample size of paeoniflorin was at a range of 0.316 5-1.899 0 μg(r=0.999 8).The average recoveries were 99.39%(RSD=0.81%).Conclusion This method is qiuckly and easy with accurate results,it can be used for the assaying and quality control of paeoniflorin in Jiangyaning capsules.