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HPLC Determination Of Hydroxysafflor yellow A in Chongderi-15 Diwan

Yuyin Li

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Abstract

Objective:Oestablisha HPLC method for the determination Of Hydroxysafflor yellow A in Chongderi-15 Diwan.Methods:Using C18 column (4.6mm×150mm,5μ).The mobile phase consisted Of methanol-water-0.7%Phosphoric Acid(26:2:72).The flowrate was 1.0 ml.min-1 with U-V detection at 403nm.Results:The liner range for the content of Hydroxysafflor yellow A was 0.1372-0.9600μg(r=1,n=7).Therecoveries of Cinnamaldehyde were 99.08%(N=9,RSD=0.7%).Conclusions:Thmethod is sample,rapid,accurate and is suitable for the determination of Hydroxysafflor yellow A in Chongderi-15 Diwan.

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What this paper is about

Objective:Oestablisha HPLC method for the determination Of Hydroxysafflor yellow A in Chongderi-15 Diwan.Methods:Using C18 column (4.6mm×150mm,5μ).The mobile phase consisted Of methanol-water-0.7%Phosphoric Acid(26:2:72).The flowrate was 1.0 ml.min-1 with U-V detection at 403nm.Results:The liner range for the content of Hydroxysafflor yellow A was 0.1372-0.9600μg(r=1,n=7).Therecoveries of Cinnamaldehyde were 99.08%(N=9,RSD=0.7%).Conclusions:Thmethod is sample,rapid,accurate and is suitable for the determination of Hydroxysafflor yellow A in Chongderi-15 Diwan.

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Available abstract

Objective:Oestablisha HPLC method for the determination Of Hydroxysafflor yellow A in Chongderi-15 Diwan.Methods:Using C18 column (4.6mm×150mm,5μ).The mobile phase consisted Of methanol-water-0.7%Phosphoric Acid(26:2:72).The flowrate was 1.0 ml.min-1 with U-V detection at 403nm.Results:The liner range for the content of Hydroxysafflor yellow A was 0.1372-0.9600μg(r=1,n=7).Therecoveries of Cinnamaldehyde were 99.08%(N=9,RSD=0.7%).Conclusions:Thmethod is sample,rapid,accurate and is suitable for the determination of Hydroxysafflor yellow A in Chongderi-15 Diwan.

Key concepts: Chromatography, Phosphoric acid, Cinnamaldehyde, High-performance liquid chromatography, Chemistry, Biochemistry, Organic chemistry, Catalysis

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