Determination of Hydroxysafflor Yellow A in Qiangjin Jiangu Powder by HPLC

Objective To establish an HPLC quantitative method for the determination of hydroxysafflor yellow A in Qiangjin Jiangu powder.Methods HPLC was used for the content determination of hydroxysafflor yellow A.The chromatography column was MUCLEODUR C 18(250 mm ×4.6 mm,5 μm) and the column temperature was 35 ℃.The mobile phase consisted of acetonitrile-0.1% phosphoric acid(11∶ 89).The flow rate was 1.0 ml·min-1and monitored at 403 nm.Results The retention time of hydroxysafflor yellow A was about 19 min.The resolution rate was more than 1.5.The regression equation for hydroxysafflor yellow A was Y = 0.033 91 X + 3.191,r = 0.9998,and the linear range was 16.00-160.0 μg·ml-1.The average recovery of hydroxysafflor yellow A was 102.6%,while RSD was 3.2%.Conclusion This method is simple,time-saving and accurate.It can be used for routine analysis of hydroxysafflor yellow A in Qiangjin Jiangu powder.