Protective Effect of Modified YinChenWuLingSan against Immunological Hepatic Injury in Model Mice

Objective To study the effects of Modified YinChenWuLingSan on immunological liver injury in mice induced by BCG+LPS and its mechanism.MethodsModified YinChenWuLingSan was given to the model mice with immunological liver injury induced by BCG+LPS.The samples of blood and liver were measured.The serum activities of ALT and AST were analyzed,organ(1iver,spleen) immunological indexes were measured and the pathological change of hepate was observed.The content of MDA and the activity of SOD in liver homogenate were measured.The content of NO and TNF-α in serum were measured also.ResultsModified YinChenWuLingSan and WuZhi pill all could significantly decrease serum ALT and AST activities of model mice,and Modified YinChenWuLingSan medium dosage had better effect than WuZhipill.Modified YinchenWulingSan was also found to inhibit the enlargerment of liver and spleen,reduce the putrescence of hepatic cell,decreased MDA content and SOD activity in liver homogenate,and reduce the levels of NO and TNF-α in serum obviously.ConclusionModified YinchenWulingSan can prevent liver damage induced by BCG and LPS in mice by scavenging oxygen free radical,inhibiting lipid peroxidation and regulating immune function.